IFNB1 antibody

Catalog No. ABIN964802

Supplier Product No.: 214-401-c40

The Rabbit Polyclonal anti-IFNB1 antibody has been validated for WB and ELISA. It is suitable to detect IFNB1 in samples from Pig.

Quick Overview for IFNB1 antibody (ABIN964802)

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFNB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IFNB1 Antibody

Supplier Product No.: 214-401-c40

Supplier: Rockland

Purpose: Interferon Beta Antibody

Cross-Reactivity (Details): This antibody is specific for swine IFN beta protein.

Characteristics: Synonyms: rabbit anti-Interferon Beta Antibody, rabbit anti-IFN-beta antibody, Interferon-beta, interferon-beta/ poIFN-beta, IFN

Purification: This product was protein A purified from monospecific antiserum by chromatography.

Immunogen:

Immunogen: This protein A purified antibody was prepared from whole rabbit antiserum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 164 amino acids of the mature swine IFN beta protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein A purified IFN-beta antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.5 kDa in size corresponding to swine IFN beta by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500-1:2,000

ELISA Dilution: 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for IFNB1

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Alternative Name: IFN beta

Background: Background: Type I interferons comprise a vast and growing group of IFN proteins. Homologous molecules to type I IFNs are found in many species, including all mammals, and some have been identified in birds, reptiles, amphibians and fish species. The mammalian types are designated IFN-alpha, IFN-beta, IFN-kappa, IFN-delta, IFN-epsilon, IFN-tau, IFN-omega, and IFN-zeta (also known as limitin). The IFN-beta proteins are produced in large quantities by fibroblasts. They have antiviral activity which is mainly involved in innate immune response. Two types of IFN-beta proteins have been described.

Gene ID: 445459

NCBI Accession: NP_001003923

UniProt: Q29412

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy, Inflammasome